Hello,
Welcome to HCM and thanks for asking your question. First of all i would like enlighten your knowledge that only one motile sperm is sufficient to fertilize ovum and make woman pregnant, no matter sex has been once or too many times.
Bleeding can be because of
menstruation or due to rupture of
hymen, because it was her 1st time. But in case of rupture hymen, high amount of bleeding does not occur generally, so in your case, i think her menstruation has started. If you want to confirm, then if bleeding continues to 3 to 4 days, then it will be surely due to menstruation.
Chances of
pregnancy are more if you have unprotected sex between 12th to 18h day of her cycle in case of regular 28 to 30 days' cycle, because only during that time, ovum is released and ready to get fertilized.
So over and all, i would like to tell you that bleeding mostly will be because of menstruation only, so you need not to worry, but from next time, always use protection.