Hi, I have been trying to conceive for a year. My blood work showed a slight elavation of prolactin which was 35. However, the nurse told me before the blood work that I didn't need to fast and that I could come in for the blood work after work, which was 4:00 in the afternoon. Could eating have effected the prolactin levels? Also, I read somewhere that prolactin levels are the highest in the morning, so if I took the test in the afternoon does that mean my levels will probably be higher since I have to retest in the morning? Also, could a prolactin level of 35 cause me to not have gotton pregnant for the past year?