Hello and Welcome to ‘Ask A Doctor’ service. I have reviewed your query and here is my advice. Masturbation doesn t lead to less sperm count. Masturbation is in fact a pleasure giving procedure. However, excess masturbation can lead to social anxiety, groin pain, lack of confidence etc. But not decreased sperm count. So don t worry about that. If you have erectile dysfunction problem than work up for that can be done like blood pressure measurement, cholesterol level checking, prostate problem, cardiac problem, smoking etc. According to cause specific treatment can be given. If needed after three day abstinence from masturbation and sex you can collect semen in sterile container and semen analysis can be done I hope I have answered your query. Let me know if you have any further questions. Regards, Dr. Parth Goswami